Home > Academics > Academic Units > Departments > Biotechnology > Amit Kumar Das
Professor
Biotechnology
Structural Biology
Prof. Das’s laboratory employs integrated structural biology, combining X-ray crystallography, protein engineering, proteomics, biophysical and biochemical methods to decipher structures and molecular mechanism of important proteins of human pathogens like Mycobacterium tuberculosis and Staphylococcus aureus.
Structure based drug designing
M. tuberculosis is one of the deadliest pathogens known to mankind. Crystal structures of important mycobacterial FAS proteins like high molecular weight ketoacylreductase (HMwFabG), HadAB heterodimeric complex, Protein tyrosine phosphatases A (PtpA), and (3R)-hydroxyacyl-CoA dehydratase (HtdX) have been solved from this laboratory. The structures based inhibitor designing and characterization against PtpA, HMwFabG and HadAB complex showed promises.
Epitope mapping
Mycobacterial Proline-rich secretary antigen (Mtc28) and mammalian cell entry proteins (Mce) and staphylococcal super antigen like proteins (SSL) have importance in host-pathogen interaction and essential for pathogenesis. Crystal structure determination, protein engineering and nanoLC coupled MALDI TOF/TOF based proteomics approaches have been employed for epitope mapping which has implementation in diagnosis and vaccination.
Enzyme Promiscuity and evolution
Probing structural insight into the substrate specificity and road map of evolution are important to address the biochemical property and molecular mechanism of an enzyme. Crystal structures of glycolytic enzymes namely GAPDH, TIM, PGK and PGM are determined to investigate the key sites/residues for biological activity. Analysis of dual specific NADP/IMP phosphatase and promiscuous SaIMPase-II revealed the evolutionary role of active site proximal loops in substrate selection.
Our future efforts will focus on mycobacterial fatty acid metabolism and virulence-associated pathway. The projects will encompass the crystal structure determination and functional characterization of the related proteins towards understanding the mechanism of activity and structure based inhibitor design.
Macroscopic amyloid fiber formation by staphylococcal biofilm associated SuhB protein by Dutta A., Bhattacharyya S. , Kundu A. , Dutta D. , Das A. K. Biophys. Chem. 32-41 (2016)
Structural elucidation of the binding site and mode of inhibition of Li+ and Mg2+ in inositol monophosphatase. by 8. Dutta A; Bhattacharyya S; Dutta D; Das AK. FEBS J 281 5309-5324 (2015)
NanoLC MALDI MS/MS based quantitative metabolomics reveals the alteration of membrane biogenesis in oral cancer by Bag S., Dutta D. , Chaudhury A. , Singh B. C., Banerjee R. , Pal M. , Paul R. R., Basak A. , Das A. K., Ray A. K., Chatterjee J. RSC Advance 62420-62433 (2016)
Reconstitution of the RNA- dependent RNA polymerase activity of Antheraea mylitta cypovirus in vitro using separately expressed different functional domains of the enzyme by Kundu A., Roychowdhury A. , Bose M. , Ghosh A. K., Das A. K. J Gen Virol. - (2016)
Crystal structure of Hexanoyl-CoA bound to β-ketoacyl reductase FabG4 of Mycobacterium tuberculosis. by 6. Dutta D, Bhattacharyya S, Roychowdhury A, Biswas R and Das AK. Biochem. J. 450 127-139 (2013)
Disruption of redox catalytic functions of peroxiredoxin-thioredoxin complex in Mycobacterium tuberculosis H37Rv using small interface binding molecules by Gurung A. B., Das A. K., Bhattacherjee A. Comput Biol Chem 69-83 (2016)
Crystal structure of dehydratase component HadAB complex of mycobacterial FAS-II pathway. by 7. Biswas R; Dutta A; Dutta D; Hazra D; Banerjee D R; Basak A; Das A K. BBRC 458 369-374 (2015)
Structure determination, mass spectrometry and epitope mapping of proteins by Das A. K. Refresher course (UGC) - (2017)
The Structures of Mycobacterial Enzymes Unclose the Artifice of N ovel Inhibitors D esigning by Das A. K. Organic Molecules: Syntheses & Applications ( OMSA) - (2017)
Enediyne-based protein capture agents: demonstration of an enediyne moiety acting as a photoaffinity label by Das J., Roy S. , Halnor S. , Das A. K., Basak A. Org Biomol Chem. 15 1122-1129 (2017)
An Insight into the Functional Mechanism of DNA Repair Helicase RecQ from Leishmania Donovani DEPARTMENT OF BIOTECHNOLOGY, MINISTRY OF SC. & TECH
A Rapid Malaria Diagnostic Test Based on External Body Fluid (SICRG) SHASTRI INDO-CANADIAN INSTITUTE
Characterization of a Putative Fatty Acid Biosynthesis Operon of Mycobacterium Tuberculosis and Lead Discovery Against the Operon Members DBT, NEW DELHI
Structure - Function Characterization of Staphylococcal B-Grasp Domain Containing Toxins and Identification of their Potential Eukaryotic Target Proteins Science and Engineering Research Board (SERB)
Studies on polyaromatic label-assisted external matrix-free mass spectrometry: exploration of a new technique for detection & estimation of small molecules and metal ions of biological interest Department of Science & Technology(DST)
Rupam Biswas
Area of Research: Structural Biochemistry
Madhuparna Bose
Area of Research: structural and molecular charcaterization mycobacterial protein
Madhurima Roy
Area of Research: Structural Biology and Bioinformatics
Debabrata Dutta
Area of Research: Structural Biochemistry
Bina Kumari Singh
Area of Research: Inhibitor design, synthesis and evaluation
Rashika Singh
Area of Research: Structural Biochemistry
Arundhoti Mandal
Area of Research: Bioorganic Chemistry
Tulika Das
Area of Research: Molecular Biology and Biophysics
