IITKGP

Responsibilities

  • Dean, Outreach

Research Areas

  • Protein protein interactions
  • Protein aggregation Studies
  • Protein Chemistry
  • Protein - small molecule interactions
Protein fibrillation/aggregation studies: Fibrils from the Aβ25-35 peptide and HSA are prepared in vitro at physiological temperature. Interactions of the monomeric and fibrillar forms of the peptides/proteins have been studied with surfactants, amino acid modifiers, osmolytes, model membranes and nanomaterials. 
 
Isolation and characterization of modified γ-crystallin (HGCc): Studies showed that the major polyphenol present in green tea, EGCG was able to prevent tryptophan oxidation of the cataractous ocular lens human γ-crystallin in presence of H2O2. The cataractous eye protein isolate has been explored for the preparation of nanoparticles and films for possible drug/compound delivery carriers and patents have been granted. Protein films prepared from the discarded protein are being tuned for specific requirements. 
 
Oxidative stress and Glycation in Proteins:  Monitoring dityrosine formation due to oxidative stress as well as the interaction of polyphenols and encapsulated polyphenols with proteins through biophysical techniques. The effect of glycation on several proteins is being investigated to monitor effects on enzymatic activity and function. Reducing sugars have shown to be responsible for oligomer formation.
 
Protein nanoparticle interactions: The protein corona formation on gold nanospheres and nanorods are being investigated. The thermodynamic aspects of the interactions as well as structural changes of the protein are being monitored for human serum albumin. Intrinsically disordered regions of the protein are found to be involved in the interactions with the nanoparticles.
 
Protein ligand binding: Studies with angiogenin indicate that the process of angiogenesis, the capacity to form blood vessels, is inhibited by polyphenols. Altered modes of inhibition are observed on encapsulation and gold nanoparticle conjugate formation. Collaborative research in the development of inhibitors for Ribonuclease A is in progress considering logic based geometric and chemical complementarity. Presently, Aromatase is under investigation for possible ways to modulate the testosterone/estradiol levels that play an important role in breast cancer.
 
  • Flavonoid loaded nanoparticles as an effective measure to combat oxidative stress in Ribonuclease A by Roy P., Parveen S. , Ghosh P. , Ghatak K. , Dasgupta S. Biochimie 162 185-197 (2019)
  • Positional preferences in flavonoids for inhibition of ribonuclease A: Where -OH where? by Tripathy D.R., Panda A., Dinda A.K., Dasgupta S. Proteins: Structure, Function and Bioinformatics 89 577-587 (2021)
  • PREPARATION OF NANOPARTICLES DERIVED FROM CATARACTOUS EYE PROTEIN ISOLATE by Dasgupta S., Chaudhury S. , Parveen S. - (2021)
  • Design of Configuration-Restricted Triazolylated -DRibofuranosides: A Unique Family of Crescent-Shaped RNase A Inhibitors. by Das A., Dasgupta S. , Pathak T. Org. Biomol. Chem 18 6340-6356 (2020)
  • Effect of Silica Nanoparticles on the Amyloid Fibrillation of Lysozyme by Konar M., Mathew A. , Dasgupta S. ACS Omega 4 1015-1026 (2019)

Co-Principal Investigator

  • Artificial Intelligence Assisted Ultrasound-guided Surgery of Non-palpable Breast Cancer and Therapy (AASTHA) INDIAN COUNCIL OF MEDICAL RESEARCH (ICMR)
  • Centre of excellence for training and research in Microfluidics IIT KHARAGPUR

Ph. D. Students

Atri Sen

Area of Research: Protein Chemistry

Kabira Sabnam

Area of Research: Protein Chemistry

Krishnananda C P

Area of Research: Nano-Bio Science

Rashmita Pan

Area of Research: biologically relevant triazoles

Suchismita Pradhan

Area of Research: Biophysical Chemistry

Sudipti Priyadarsinee

Area of Research: Biophysical Chemistry

Sujan Santra

Area of Research: Protein Chemistry

Tridib Samanta

Area of Research: Protein Chemistry